188体育

188体育-www.bncc.org.cn北纳生物

一文了解蛋白质常见的四种测定方法

发布时间:2020-02-19 16:35 作者:潘东
0

DANBAIZHISHIYOUANJISUANYI“TUOSHUISUOHE”DEFANGSHIZUCHENGDEDUOTAILIANJINGGUOPANQUZHEDIEXINGCHENGDEJUYOUYIDINGKONGJIANJIEGOUDEWUZHI。DANBAIZHIZHONGYIDINGHANYOUTAN、QING、YANG、DANYUANSU,CHANGJIANDEDANBAIZHIJIANCEFANGFAHENDUO。DANBAIZHIHANLIANGCEDINGSHIZHITONGGUOWULIHUOHUAXUEFANGFADUIDANBAIZHIHANLIANGJINXINGCEDING,ZHUYAOYOUYIXIA4ZHONG。

1.凯氏定氮法

ZHUNBEI4GE50mLKAISHISHAOPINGBINGBIAOHAO,XIANG1、2HAOSHAOPINGZHONGJIARUDINGLIANGDEDANBAIZHIYANGPIN,LINGWAILIANGGESHAOPINGZUOWEIDUIZHAO,ZAIMEIGESHAOPINGZHONGJIARULIUSUANJIA-LIUSUANTONGHUNHEWU,ZAIJIARUNONGLIUSUAN,JIANG4GESHAOPINGFANGDAOXIAOHUAJIASHANGJINXINGXIAOHUA,ZHIHOUJINXINGZHENGLIU,QUANBUZHENGLIUWANBIHOUYONGBIAOZHUNYANSUANDIDINGGESHAOPINGZHONGSHOUJIDEANLIANG,ZHIZHIZHISHIJIHUNHEYEYOULVSEBIANHUIDANZIHONGSE,JIWEIDIDINGZHONGDIAN,JIESUANCHUDANBAIZHIHANLIANG。

2.双缩脲法

SHUANGSUONIAOFASHIGEYONGBISEFACEDINGDANBAIZHINONGDUDEFANGFA,LIUANBUGANRAOXIANSE, Cu2+YUDANBAIZHIDETAIJIAN,YIJILAOANSUANCANJILUOHE,XINGCHENGZILANSELUOHEWU,CIWUZAI540nmBOZHANGCHUYOUDAXISHOU。SHOUXIANLIYONGBIAOZHUNDANBAIRONGYEHESHUANGSUONIAOSHIJIHUIZHIBIAOZHUNQUXIAN,JIANGDAICEXUEQINGYULIUSUANNAZAIDAICESHIGUANZHONGHUNHE,BINGYONGZHIJIARULIUSUANNABUHANXUEQINGDESHIGUANZUOWEIDUIZHAO,JIANGLIANGZHISHIGUANJIARUDENGLIANGDESHUANGSUONIAOSHIJI,CHONGFENHUNHEHOUYU37℃HUANJINGZHONGFANGZHI10FENZHONG,ZAI540nmBOZHANGJINXINGBISE,YIDUIZHAOGUANDIAOLING,DUQUXIGUANGDUZHI,YOUBIAOZHUNQUXIANSHANGZHIJIECHACHUDANBAIZHIHANLIANG。SHUANGSUONIAOFACHANGYONGYU0.5g/L~10g/LHANLIANGDEDANBAIZHIRONGYECEDING。

3.酚试剂法

QU6ZHISHIGUANFENBIEBIAOHAO,QIAN5ZHISHIGUANFENBIEJIARUBUTONGNONGDUDEBIAOZHUNDANBAIRONGYE,HOUYIZHISHIGUANJIADAICEDANBAIZHIRONGYE,BUJIABIAOZHUNDANBAIRONGYE,MEIZHISHIGUANYETIZONGLIANGTONGGUOJIARUZHENGLIUSHUIBUZUERBAOCHIYIZHI,JIANGYETIHUNHEJUNYUN,ZAISHIWENXIAFANGZHI30FENZHONG,YIWEIJIADANBAIZHIRONGYEDEZHISHIGUANZUOWEIKONGBAIDUIZHAO,YU650nmBOZHANGCHUCEDINGGEGUANZHONGRONGYEDEXIGUANGDUZHI

4.紫外吸收法

188体育DADUOSHUDANBAIZHIZAI280nmBOZHANGCHUYOUTEZHENGDEDAXISHOU,ZHEISHIYOUYUDANBAIZHIZHONGYOULAOANSUAN,SEANSUANHEBENBINGANSUANCUNZAI,KEYONGYUCEDING0.1~0.5mg/mLHANLIANGDEDANBAIZHIRONGYE。QU9ZHISHIGUANFENBIEBIAOHAO,QIAN8ZHISHIGUANFENBIEJIARUBUTONGNONGDUDEBIAOZHUNDANBAIRONGYE,1HAOSHIGUANBUJIABIAOZHUNDANBAIRONGYE,HOUYIZHISHIGUANJIADAICEDANBAIZHIRONGYE,ERBUJIABIAOZHUNDANBAIRONGYE,MEIZHISHIGUANYETIZONGLIANGTONGGUOJIARUZHENGLIUSHUIBUZUERBAOCHIYIZHI,JIANGYETIHUNHEJUNYUN,ZAI280nmBOZHANGCHUJINXINGBISE,JILUXIGUANGDUZHI。

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